1. How much iron is there in the hemoglobin of a 60 kg adult? Assume the blood volume is 70 ml/kg and that the hemoglobin content of blood is 16 g/100 ml.
2. The equilibrium constant K for the binding of oxygen to myoglobin is 1 x 10-6. The rate constant for the combination of O2 with myoglobin in 2 x 107 M-1s-1.
3. What effect does each of the following treatments have on the oxygen affinity of hemoglobin A (Adult hemoglobin) in vitro?
4. The pKa of an acid depends upon its environment. Predict the effect of the following changes on the pKa of the glutamic acid side chain.
5. An oligomeric protein has a molecular weight of 72,400 daltons. It is a manganese protein, with 0.076% manganese (the atomic weight of Mn is 54.94) When 868 mg of the protein is reacted with 1-fluoro-2,4-dinitrobenzene and the peptide bonds completely hydrolyzed with 6N HCl at 100 C overnight, 2.86 mg of the 2,4 dinitro derivative of threonine was produced and 3.3 mg of the 2,4 dinitro derivative of methionine was also produced.
6. Keratin is the major protein in wool from sheep. On average, sheep wool grows 60 cm per year. The fundamental structure of wool is keratin, a rope like protein that consists primarily of alpha helices. The alpha helix contains 3.6 amino acids per turn with a rise of 0.56 nm per turn. (In other words each 0.56 nm of hair contains 3.6 amino acids.) The rate determining step for hair growth is the formation of peptide bonds in the keratin chains. Calculate the rate of peptide bond formation in the keratin chains that would correspond to the observed yearly hair growth.
7. Many enzymes must bind two substrates to catalyze the formation of a product. Often the binding of one substrate will affect the binding of the other substrate.
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